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Clathrin coated vesicles

What is clathrin-mediated endocytosis? MBInf

A. Clathrin is a triskelion shaped scaffold protein. B. Clathrin assembles to form a coat around a mature vesicle. C. Formation of the clathrin coated vesicle requires changes in membrane curvature, which is driven by PIP2 levels and BAR protein binding In the final stages of clathrin-coated vesicle (CCV) formation, Phosphatidylinositol-4,5-bisphosphate (PIP2) undergoes a dephosphorylation by phosphatases such as synaptojanin 1 (Synj1). This promotes further membrane curvature at the vesicle bud, dissociation of the BAR domain proteins (BDPs) and closing of the membrane bud neck. Read more.

How are clathrin-coated vesicles uncoated? MBInf

  1. Clathrin coated vesicles (CCVs) mediate the vesicular transport of cargo such as proteins between organelles in the post-Golgi network connecting the trans-Golgi network, endosomes, lysosomes and the cell membrane
  2. ent form of traffic from the plasma membrane to endosomes (endocytosis), a pathway by which ligands such as hormones, transferrin, immunoglobulins, LDL (low-density lipoprotein), viruses, and their receptors enter cells
  3. Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts. Pastan IH. Clathrin-coated vesicles: isolation.
  4. These indentations are known as clathrin-coated pits; see RECEPTOR-MEDIATED ENDOCYTOSIS. clathrin a highly conserved, fibrous protein, mol. wt 180,000, which forms a characteristic polyhedral coat on the surface of coated pits and coated vesicles
  5. Clathrin definition, a basketlike network of protein molecules that forms on the cell membrane in response to the attachment of ligands to receptors and becomes the inside surface of the coated vesicle during endocytosis
  6. 3.2 Formation of clathrin and COP-coated vesicles. The structure of clathrin and clathrin-coated vesicles is known in some detail. Clathrin consists of a heavy chain of M r 180,000 together with a light chain of M r 35,000. Clathrin molecules successively assemble into a polyhedral, cage-like coat on the surface of the coated pit

Clathrin-coated vesicle - uniprot

Forty Years of Clathrin-coated Vesicles. Robinson MS(1). Author information: (1)Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK. The purification of coated vesicles and the discovery of clathrin by Barbara Pearse in 1975 was a landmark in cell biology AP2 is the only heterotetrameric AP involved in clathrin-coated vesicle formation at the plasma membrane and binds to membranes without the need of a small GTPase because it binds PI(4,5)P 2. Other heterotetrameric APs that assemble clathrin-coated vesicles on other membranes require Arf GTPases

Clathrin-mediated endocytosis is mediated by the production of small (approx. 100 nm in diameter) vesicles that have a morphologically characteristic coat made up of the cytosolic protein clathrin. Clathrin-coated vesicles (CCVs) are found in virtually all cells and form domains of the plasma membrane termed clathrin-coated pits 1. Clathrin-coated vesicles Clathrin-coated vesicles (CCVs) are one of four types of coated membrane carriers [coat protein I (COPI)-coated vesicles, coat protein II (COPII)-coated vesicles, retromer-coated vesicles, and CCVs] that are responsible for intracellular protein transport events in eukaryotic cells [65-67] Clathrin-mediated endocytosis This animation shows the process of clathrin-mediated endocytosis of transferrin receptors, focusing on the assembly and disassembly of the clathrin cage. A single. CLATHRIN COATED VESICLES eClathrin is also a protein which is involved in the coated vesicle formation e Clathrin associates with different adaptor proteins which forms vesicles that transports either from plasma membrane to endosome or from trans-Golgi network to lysosome

Clathrin-Coated Vesicle - an overview ScienceDirect Topic

  1. ation of the structure of a number of CCV coat components at molecular resolution and the identification of novel regulatory proteins that control CCV formation in the cell. In addition, pathways of (a) phosphorylation, (b) recepto
  2. Clathrin-coated vesicles were the first discovered and remain the most extensively characterized transport vesicles. They mediate endocytosis of transmembrane receptors and transport of newly synthesized lysosomal hydrolases from the trans-Golgi network to the lysosome
  3. If a vesicle pinches off an endomembrane and fuses with the plasmalemma, is its content exported out of the cell, the process is called exocytosis. Exocytotic vesicles are usually not clathrin coated, most of them have no coat at all. It seems as if microtubuli participate in the intracellular transport of coated vesicles
  4. Clathrin‐coated vesicles carry proteins and lipids between compartments of eukaryotic cells. Best‐studied is their role in receptor‐mediated endocytosis and in the transport of lysosomal proteins from the trans‐Golgi network to the endosomal system
  5. Clathrin-coated vesicles have been implicated in several distinct intracellular transport steps. In particular, the internalisation of plasma membrane proteins by receptor-mediated endocytosis (RME) and the transport of lysosomal enzymes from the TGN [ 2-4 ]

Clathrin and coated vesicles

  1. Clathrin, moving proteins and lipids about . Clathrin-coated vesicles are found in all eukaryotic cells, but they are particularly enriched in the brain, where they play a major role in the formation of neurotransmitter-containing pre-synaptic vesicles required for synaptic nerve transmission
  2. Clathrin-coated vesicles, identified by negative staining with uranyl acetate, were purified from Chlamydomonas reinhardtii. Isolated coated vesicles had diameters ranging from 70 to 140 nm (mean.
  3. - uses Clathrin coated vesicles List all the steps of the formation and degradation of an endocytic vesicle. - binding of the extracellular molecule (ligand) to specific receptor on the cell surfac
  4. Clathrin is the main constituent of the polygonal network that forms the coat of coated vesicles and coated pits. In all cell types, clathrin-coated vesicles are responsible for receptor-mediated endocytosis at the plasma membrane and for the receptor-mediated sorting of lysosomal enzymes from trans-Golgi membranes to a pre-lysosomal compartment
  5. Ultrastructural analysis of synaptic vesicle recycling reveals that clathrin is not required for the initial rapid step of vesicle recycling by ultrafast endocytosis at the plasma membrane and.

Clathrin definition of clathrin by Medical dictionar

Molecular movie representing the assembly of a clathrin coated pit, its dynamin-based budding to form a coated vesicle and the removal of the coat mediated by the uncoating Hsc70/auxilin dependent. -Dymanin only involved in clathrin coated vesicles-Contains a PI(4,5)P2 binding domain, which tethers the protein to the membrane by binding to modified phosphoinositols-Also contains a GTPase domain, which pinches off and squeezes vesicle so it can bud Clathrin‐coated vesicles are short lived within the cytosol that contains uncoating proteins like Hsc70, and it was thus not surprising that these transient intermediates were only rarely seen in our vesicle preparations

Clathrin-coated endocytic vesicles are produced by a complex modular protein machinery that transiently assembles on the plasma membrane. This machinery selects and concentrates cargo molecules. Clathrin-coated vesicle cycle. Transport vesicles are classified according to the components of the protein coat that surrounds them during their genesis and early life. One of the most common and probably best-characterized classes of coated vesicle is that comprising three-layered Clathrin-coated vesicles (CCVs) Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein.

Clathrin Definition of Clathrin at Dictionary

Clathrin‐coated vesicles (CCVs) are an important class of transport organelles that mediate the endocytosis of proteins and lipids at the plasma membrane and the transport of proteins from the trans‐Golgi network to the endosomal/lysosomal system The nature of the protein coat on clathrin-coated vesicles and the interactions responsible for the structure and reformation of clathrin baskets have been investigated. Coated vesicles were isolated from bovine brain using a rapid, one-day modification of the method of Pearse (1975). Th This vesicle transport lecture explains the mechanism of vesicle formation and release of transport vesicles in cellular transport pathway. http://shomusbiol.. Molecular mechanism and physiological functions of clathrin‑mediated endocytosis Harvey T. McMahon and Emmanuel Boucrot Abstract | Clathrin-mediated endocytosis is the endocytic portal into cells through which cargo is packaged into vesicles with the aid of a clathrin coat. It is fundamental t Clathrin-coated vesicles that bud from the PM could be involved in PM-to-Golgi transport because many recycling proteins are internalized from the PM in these structures. However, previous investigations of this problem by us and others have failed to support a role for coated vesicles(27, 28, 29). To test directly the role of clathrin-coated.

3.2 Formation of clathrin and COP-coated vesicles - OpenLear

Introduction. Clathrin-coated vesicles (CCVs) are important transport intermediates in all eukaryotic cells. Their coats consist of two major components: clathrin, which provides a stabilizing scaffold, and heterotetrameric adaptor protein (AP) complexes, which attach the clathrin to the membrane and select the vesicle cargo (Robinson, 2004) populations of clathrin-coated vesicles isolated from cells. We have therefore used cryo-electron tomography to study the lattice organization of individual clathrin-coated vesicles and the disposition of the captured vesicle with respect to the surrounding coat. We find a wide range o

The point of divergence between these types of MVEs is drawn at early endosomes, but the existence of distinct early endosomes feeding into these two pathways cannot be excluded. Red spots symbolize clathrin associated with vesicles at the plasma membrane (clathrin-coated vesicles [CCV]) or bilayered clathrin coats at endosomes Topics of the 21 papers include the role of microtubules in transport, kinases- regulating Golgi apparatus structure and functions, N-glycans as apical targeting signals, aspects of dominant distal renal tubular acidosis, regulation of clathrin-coated vesicles and imaging of clathrin-based endocytosism, membrane traffic to and from lysosomes, lipid rafts in protein interaction and signaling. Our hypotheses are that normal huntingtin is required for the internalization of clathrin-coated vesicles and transport of recycling vesicles and that mutant huntingtin interferes with vesicle budding from the plasma membrane and from recycling endosomes.Aim 1 examines how normal huntingtin contributes to endocytosis and subsequent vesicle. Clathrin-Coated Vesicles is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings).Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity What uses a clathrin-coated vesicle? Molecules brought into a cell via receptor-mediated endocytosis are carried to their destination in the cell in clathrin coated vesicles

Definition of clathrin-coated vesicles in the Definitions.net dictionary. Meaning of clathrin-coated vesicles. What does clathrin-coated vesicles mean? Information and translations of clathrin-coated vesicles in the most comprehensive dictionary definitions resource on the web Clathrin-coated vesicles were isolated from bovine brains and tested for binding to calmodulin conjugated to Sepharose 4B. In the experiments using clathrin-coated vesicles, the presence of disassembled clathrin in the vesicle preparations was reduced by centrifugation at 100,000 × g. After discarding the supernatant that contained unassembled. Uncoating of Clathrin-Coated Vesicles by Uncoating ATPase from Developing Peas' Thomas Kirsch and Leonard Beevers* Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 7301 9 A cytosolic uncoating ATPase (an enzyme that dissociates clath- rin from clathrin-coated vesicles in the presence of ATP) wa

Video: Forty Years of Clathrin-coated Vesicles

Best Answer: Clathrin is a protein that is the major constituent of the 'coat' of the clathrin coated pits and coated vesicles formed during endocytosis of materials at the surface of cells. Clathrin molecules are recruited to a membrane segment that is destined to be incorporated into a vesicle 26. An important difference between clathrin coated vesicles and COP I coated vesicles is: a) COPI coated vesicles contain ARF and clathrin coated vesicles do not contain ARF. b) COPI coated vesicles contain AP-1 and clathrin coated vesicles do not contain AP-1

Clathrin - an overview ScienceDirect Topic

A partially invaginated membrane structure (bud or pit) involved in receptor-mediated endocytosis consisting of a cluster of receptor proteins attached on the cytosolic side by means of adaptin molecules to the protein clathrin, which forms a lattice-shaped coating In eukaryotic cells, receptor-mediated endocytosis and exit of specific trans-Golgi network cargo proteins (newly synthesized lysosome enzymes and secretory proteins) occur by way of clathrin-coated pits and clathrin-coated vesicles (CCVs). The major structural proteins of the coat are the heavy and light chains of clathrin Visualization of Clathrin-coated Vesicles by Cryo-electron Tomography - Volume 13 Issue S02 - Y Cheng, W Boll, T Kirchhausen, S Harrison, T Wal Receptor-mediated endocytosis by clathrin-coated vesicles: evidence for a dynamic pathway. Jeffrey L Salisbury , J. S. Condeelis, N. J. Maihle, P. Satir Research output : Contribution to journal › Articl

vesicles, where they provide the driving force for the uptake of various neurotransmitters (Moriyama et al., 1992). Following the fusion of synaptic vesicles at the nerve terminal, clathrin-coated vesicles function in the retrieval of proteins (including presumably the V-ATPase) from the presynaptic membrane Examples of clathrin-coated pits at various degrees of invagination (A-D); E shows an apparently free, clathrin-coated vesicular profile that, however, may be surface-connected in another plane of sectioning. The clathrin coat is indicated by arrowheads in A and B. The small arrows in C and D indicate the necks connecting the pits to the. An activator of the clathrin-coated vesicle proton translocating ATPase has been purified 1600-fold from bovine brain. The activator, which requires detergent (polyoxyethylene 9-lauryl ether) for release from clathrin-coated vesicles, is heat-stable, trypsin-sensitive, and has an apparent molecular mass of about 6 kDa as determined by high performance liquid chromotography Bottom panel shows the reverse co-IP of mouse retinal extract using anti-Clathrin antibody to pull down REEP6. (D) EM and Immuno-EM images of Clathrin coated vesicles purified from bovine retina. The top panels show Clathrin-coated vesicles with the characteristic triskelion architecture

Endocytosis - Wikipedi

Many molecules enter the cell interior within clathrin-coated vesicles, in process termed endocytosis. This process is critical to the way we move and think. At the tip of each axon, synaptic vesicles (packages of neurotransmitter) release their contents when the nerve is stimulated by fusing with the cell surface Endocytosis can occur at a clathrin-coated pit or at other regions of the membrane. The endocytic vesicles will fuse with early endosomes. Some membrane proteins and lipids will be recycled back to the plasma membrane via vesicles derived from the early endosome The pit can accommodate ~ 1000 proteins. Polymerization of clathrin drives formation of a vesicle that eventually pinches off from the plasma membrane. The GTPase dynamin catalyzes the pinching off reaction. The clathrin-coated vesicles fuse with endosomes where the low pH dissociates ligands from receptors Clathrin . Clathrin Adaptors . Clathrin coats contain both clathrin and clathrin adaptor proteins. While clathrin acts as the scaffold, the clathrin adaptors bind to protein and lipid cargo. Specific cargos are recruited into clathrin-coated vesicles with the aid of CLASP proteins (clathrin-associated sorting proteins), such as ARH and Dab2 the formation of clathrin-coated vesicles at the plasma membrane. The role of the three major identified coat proteins, AP-2, clathrin and dynamin, is now well characterized. The AP-2 complex plays a central role in both the organization and function of plasma membrane coated pits: it drives clathrin assembly onto the plasma membrane and.

the interaction between clathrin-coated vesicles and the actin cytoskeleton is mediated by antigens identified by monoclonal antibody A-7Cll. Clathrin-coated vesicles (CCV) associated with the plasma membrane participate in the clustering and internalization of receptor-ligand complexes [l, 21. Those associated with th Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules The denser peak overlaps with the major distribution of clathrin and represents a region of the gradient enriched in coated vesicles (cv). Further purification of the pooled cv-fractions has shown that these PM marker activities are not borne by the cv, but are instead carried by smooth membrane fragments also present in these fractions Gα-interacting protein (GAIP) is a member of the RGS (regulators of G protein signaling) family, which serve as GAPs (GTPase-activating proteins) for Gα subunits. Previously, we demonstrated that GAIP is localized on clathrin-coated vesicles (CCVs). Here, we tested whether GAIP-enriched vesicles could accelerate the GTPase activity of Gα i.

In vitro protein interaction assays revealed that AtECA1 binds to the clathrin H chain via its carboxyl-terminal domain. These results suggest that these AP180 amino-terminal homology/epsin amino-terminal homology domain-containing proteins, AtECA1, AtECA2, and AtECA4, may function as adaptors of clathrin-coated vesicles budding from the cell. Clathrin-coated vesicles contain a proton translocating ATPase which operates in parallel with a chloride transporter (Xie, X.-S., Stone, D.K., and Racker, E. (1983) J. Biol. Chem. 258, 14834-14838). The polypeptide antibiotic, duramycin, has a dual inhibitory effect on clathrin-coated vesicle acidification GAIP Is Localized on Clathrin-coated Buds or Vesicles Located Near the TGN in Pituitary Cells. When GAIP was localized by immunofluorescence in AtT-20 cells (Figure 4A) and semithin cryosections of rat pituitary (Figure 4B), punctate staining for GAIP was distributed throughout the cytoplasm but was concentrated in the Golgi region Clathrin Coated Vesicle listed as CCV. Clathrin Coated Vesicle - How is Clathrin Coated Vesicle abbreviated? Clathrin-coated vesicles; Clathrin-coated vesicles The intracellular vesicle transport. The structural and metabolic stability of a eukaryotic cell depends on the trafficking of material between sub-cellular compartments. Intracellular trafficking requires the formation and correct targeting of small, membrane-bound vesicles. Vesicles are first created from a so-called donor compartment

purification of clathrin-coated vesicles from bovine brain, liver, adrenal gland in isolation plasma membrane, organelles, and cellular structures of organelles and cellular structure NCCV - Non-Clathrin-Coated Vesicle. Looking for abbreviations of NCCV? It is Non-Clathrin-Coated Vesicle. Non-Clathrin-Coated Vesicle listed as NCCV

Clathrin-Coated Vesicles Klatrintäckta vesiklar Svensk definition. Blåsor som bildas när höljeförsedda gropar i cellmembranet tränger in och knoppas av. Ytan på dessa blåsor är täckt av ett fackverksliknande nät av proteinet klatrin Internal Medicine; The Eugene McDermott Center for Human Growth and Development; Eugene Mcdermott Center For Human Growth & Developmen The DnaJ protein auxilin has been extensively studied in vitro as a cofactor for uncoating clathrin-coated vesicles by the chaperone Hsc70. Recent studies provide the first evidence that auxilin plays this role in vivo, and work on a new mammalian auxilin suggests the protein may have more complex cellular functions

Abstract. yeast Clathrin-coated vesicles serve a number of functions in animal cells. They function as the site of ligand-receptor clustering during the process of receptor-mediated endocytosis and also serve in targeting of newly synthesized lysosomal enzymes from the Golgi complex to lysosomes (Mellman, 1996) clathrin-coated vesicles in which many Arf GAP proteins are involved (see Table 1 for each Arf GAP subtype). This review will investigate the possibility that there is an analogy between a role for ArfGAP1 in COPI-vesicle formation and that of Arf GAP protein in clathrin-vesicle formation. However, compare Clathrin-Coated Vesicles definition, categories, type and other relevant information provided by All Acronyms. CCV stands for Clathrin-Coated Vesicles The purification of coated vesicles and the discovery of clathrin by Barbara Pearse in 1975 was a landmark in cell biology. Over the past 40 years, work from many labs has uncovered the molecular details of clathrin and its associated proteins, including how they assemble into a coated vesicle and how they select cargo Clathrin-coated vesicles are also involved in protein sorting at the trans-Golgi network (Traub, 2005), and the assembly of the Golgi apparatus itself requires clathrin (Radulescu et al., 2007). This functional diversity requires assembling polymorphic scaffolds that are able to accommodate large variations in the size, shape, and molecular.

Instead, increased PtdIns(4,5) P 2 was found in CCVs (clathrin-coated vesicles), which proliferated several-fold in mass within 60 min of hyperosmotic stress, according to the abundance of CCV-associated proteins and lipids Clathrin-coated vesicles isolated from calf brain contain an ATP-dependent proton pump. Proton movement was monitored by measuring [14C]methylamine distribution. Addition of Mg2+ and ATP to coated vesicles equilibrated with [14C]methylamine resulted in the generation of a 4- to 5-fold concentration gradient, corresponding to a delta pH of 0.6-0. Intracellular transport, sorting, and turnover of acetylcholinesterase. Evidence for an endoglycosidase H-sensitive form in Golgi apparatus, sarcoplasmic reticulum, and clathrin-coated vesicles and its rapid degradation by a non-lysosomal mechanis Clathrin-Coated Vesicles (n.) 1. Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES

Clathrin mediated endocytosis - cell proccess

Clathrin-coated vesicles bud from eukaryotic plasma membrane fragments when adaptins, clathrin, and dynamin-GTP are added. A. adaptins were omitted B. Clathrin was omitted C. Dynamin was omitted D. Prokaryotic membrane fragments were used. Viruses are the ultimate scavenger—a necessary consequence of their small genomes Enrichment of Presenilin 1 Peptides in Neuronal Large Dense-Core and Somatodendritic Clathrin-Coated Vesicles

Clathrin-coated vesicles transport selected sets of membraneassociated macromolecules between subcellular compartments (6, 10, 13, 25-30). Isolated coated vesicles have offered fruitful opportunities for structural study (3, 7, 8, 11, 12, 14 Assembly of clathrin-coated pits and their maturation into coated vesicles requires coordinated interactions between specific lipids and several structural and regulatory proteins. In the presence of primary alcohols, phospholipase D generates phosphatidylalcohols instead of PA, reducing stimulation of phosphatidyl inositol 5

ABSTRACT AP50 is the 50,000-dalton protein component found in clathrin-coated vesicles as part of the coat assembly protein (AP) complex, AP-2. AP50 cDNA clones were isolated from rat brain cDNA libraries, and their nucleotide sequence was determined uncoating of clathrin-coated vesicles (CCVs) during et al., 2000; Lemmon, 2001). Mammalian isoforms in-neuronal endocytosis. We identified two peptides that clude the neuron-specific auxilin 1 and the ubiquitously inhibit the ability of Hsc70 and auxilin to uncoat CCVs expressed auxilin 2 (also called G-cyclin associated ki Proc. Natl. Acad. Sci. USA Vol. 96, pp. 6722-6727, June 1999 Cell Biology Clathrin-coated vesicles bearing GAIP possess GTPase-activating protein activity in vitro (heterotrimeric G proteinsyRGS proteinsyGAP) THIERRY FISCHER, ERIC ELENKO, J. MICHAEL MCCAFFERY, LUC DEVRIES, AND MARILYN GIST FARQUHAR* Division of Cellular and Molecular Medicine and Department of Pathology, University of. To test if clathrin was required for the formation of The small GTP binding protein ARF has been impli- these vesicles, a sla2 chc1-5 strain was constructed cated in budding clathrin-coated vesicles (CCVs) from and examined by EM in the same fashion. Remarkably, Golgi and endosomal membranes Clathrin coated pits form in the cell membrane and have a very short life- almost immediately they pinch off into a clathrin coated vesicle which invaginates and pinches off from the cell. Caveola specialized lipid environments, that have specific cargo for endocytosis in animal

You are studying the role of dynamin in the budding and pinching off mechanism of clathrin coated vesicles from the plasma membrane. You know that dynamin uses GTP hydrolysis to perform its function, but you would like to test how dynamin behaves in the presence of a non-hydrolyzable analog of GTP 4 °C. About 50% of the vesicles in these samples corre-spond to clathrin-coated vesicles as determined by nega-tive staining and electron microscopy (not shown). Aliquots of the resulting fractions were mixed with Laemmli sample buffer in the presence of b-mercap-toethanol, boiled for 2 minutes and processed for West-ern blot analysis [13] The animation will need an update, because something new was reported about clathrin-coated vesicles (CCV) and the pits (CCP) that form when the membrane invaginates to bring cargo in from outside. Another EMBL team, also reporting in Science, found that clathrin is more gymnastic than previously recognized The cages have point group symmetries and discrete size distributions , whereas in vivo formed clathrin-coated vesicles are surrounded by cages that can deviate from point group symmetry . In both cases, each cage subunit makes the same set of interactions with the same number of partners

Video: Clathrin coated vesicles - Unacadem

Requirement for Membrane Remodeling in the Budding of AP-1/clathrin-coated vesicles from the Golgi complex Christina Snider Research Advisor: Todd Graham Department of Biological Sciences Vanderbilt University April 23, 201 We found auxilin-1 uncoated PI3P containing clathrin coated vesicles more efficiently than auxilin-2, and the reverse to be the case for PI4P clathrin coated vesicles (Manuscript in preparation). Lab Collaborators on this project are: Kangmin He, Ben Capraro, Iris Rapaport, Kevin Bu Sorting SNAREs into clathrin-coated vesicles The protein and phospholipid composition of cellular membranes defines the identity of that membrane; what binds to it and what reactions occur both on its surface and inside the cavity it surrounds

Clathrin-coated vesicles carry traffic from the plasma ous increase in the dynamin signal (Rappoport and Si-membrane to endosomes. We report here the real-mon, 2003). time visualization of cargo sorting and endocytosis by Do these observations on selected coated pits repre-clathrin-coated pits in living cells In fixed cells (0 min), the antibody binds to the cell surface and is found in some clathrin-coated pits. In cells that have been incubated at 37°C for 15 min after antibody binding, the label is found in large 'empty' vesicles, which probably correspond to early endosomes. After one hour at 37°C Clathrin-coated vesicles from brain are primarily involved in synaptic vesicle recycling and are substrates for the constitutively expressed heat shock cognate hsc70 protein (uncoating ATPase). To investigate the regulation of clathrin coat turnover in other tissues the activity of hsc70 towards coated vesicles from other sources was examined Cell Biology Class Test Questions for Exam #3. 1. Name two similarities and two differences between the cellular processes of importing protein into the ER and importing protein to the nucleus. 2. What proteins are directly involved in the transportation of cargo in a clathrin-coated vesicle? a. Dynamin b. Adaptins c. Cargo receptors d. t SNARES 3

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